غربال ارقام توت فرنگی متحمل به سرمای زمستانه براساس برخی شاخص های فیزیولوژیکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم باغبانی دانشکده کشاورزی دانشگاه بوعلی سینا، همدان

2 استاد گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه بوعلی سینا، همدان

3 دانشیار گروه علوم باغبانی دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان

4 دانشیار گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی، دانشگاه کردستان، سنندج

چکیده

به منظور بررسی تحمل به سرمای زمستانه هفت رقم توت­فرنگی، آزمایشی به­صورت آزمایش کرت­های خرد شده در قالب طرح بلوک­های کامل تصادفی، در دانشکده کشاورزی دانشگاه بوعلی سینا در سال 1393 انجام شد. بعد از اعمال تیمار دماهای پایین (4+، 5-، 10-، 15-، 20- و 25- درجه سانتی­گراد)، درصد نشت یونی برگ، شاخص خسارت یخ­زدگی، محتوی آب نسبی برگ، غلظت کلروفیل برگ و مقادیر نسبی پارامترهای فلورسنس کلروفیل سنجیده شد. اثر تیمار دمای پایین، رقم و برهم­کنش آنها بر همه صفات مورد ارزیابی در سطح احتمال یک درصد معنی­دار شد. نتایج مقایسه میانگین­ها نشان داد دامنه اختلاف ارقام برای صفات مورد ارزیابی در دمای 25- درجه سانتی­گراد بسیار بیشتر از سایر تیمارهای دمایی بود. همبستگی بالایی بین شاخص­های ارزیابی میزان تحمل ارقام به دمای پایین وجود داشت. ارقام ‘کارسنبرگ’ و ‘کویین الیزا’ با کمترین مقادیر درصد نشت یونی و درصد خسارت یخ­زدگی و بیشترین مقادیر کلروفیل و مؤلفه­های فلورسنس کلروفیل در دمای 25- درجه سانتی­گراد، به­ترتیب بیشترین تحمل به سرما را نشان دادند. کمترین تحمل به سرما، در ارقام ‘چاندلر’ و ‘تنسی بیوتی’ با بیشترین درصد نشت یونی و درصد خسارت یخ­زدگی و کمترین مقادیر کلروفیل و مؤلفه­های فلورسنس کلروفیل در دماهای پایین مشاهده شد.

کلیدواژه‌ها


عنوان مقاله [English]

Screening of winter cold tolerant strawberry cultivars based on physiological indices

نویسندگان [English]

  • Farhad Karami 1
  • Mansour Gholami 2
  • Ahmad Ershadi 3
  • Adel Sio Se Mardeh 4
1 Ph.D. Student, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2 Professor, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
3 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
4 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Kurdistan University, Sanandaj, Iran
چکیده [English]

In this study, the winter cold tolerance of seven strawberry cultivars was evaluated using a RCBD design with a split plot arrangement at Bu-Ali Sina University in 2014. After exposure of plants to low temperatures (+4, -5, -10, -15, -20 and -25oC), the values of electrolyte leakage, index of freeze injury, RWC, total chlorophyll and chlorophyll fluorescence parameters (F0, Fm, Fv and Fv/Fm) were measured. Low temperature, cultivar and their interactions had significant effects (P≤0.01) on all measured parameters. Means slicing of traits showed that genotypic differences were most obviously identified in -25oC as compared to the other temperature treatments. There was strong correlations between the evaluation methods for screening winter cold tolerant cultivars. The strawberry `Karssenberg' and `Queen Elisa' were the most cold tolerant cultivars with the lowest electrolyte leakage and freeze injury index. These cultivars had the highest chlorophyll content and chlorophyll fluorescence rates in -25oC whereas `Chandler' and `Tennessie Beauty' with the highest electrolyte leakage and freeze injury index values, the lowest chlorophyll content and chlorophyll fluorescence rates in low temperatures, were the most susceptible cultivars to low temperatures.

کلیدواژه‌ها [English]

  • Chlorophyll fluorescence
  • electrolyte leakage
  • freezing injury
  • Screening
  • strawberry
1 . میرمحمدی میبدی ع م و ترکش اصفهانی س (1383) مدیریت تنش­های سرما و یخ­زدگی گیاهان زراعی و باغی. انتشارات جهاد دانشگاهی واحد صنعتی اصفهان. اصفهان. 312 ص.

 

2 . Azzarello E, Mugnai S, Pandolfi C, Masi E, Marone E and Mancuso S (2009) Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees. 23(1): 159-167.

3 . Baker NR and Rosenqvist E (2004) Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany. 55(403): 1607-1621.

4 . Berova M, Zlatev Z and Stoeva N (2002) Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulg. Plant Physiol. 28(1-2): 75-84.

5 . Bilger W, Schreiber U and Lange O (1987) Chlorophyll fluorescence as an indicator of heat induced limitation of photosynthesis in Arbutus unedo L. Plant Response to Stress. Pp. 391-399.

6 . Björkman O and Demmig B (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta. 170(4): 489-504.

7 . Campos PS, Quartin V, Ramalho J and Nunes MA (2003) Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. plants. Journal of Plant Physiology. 160(3): 283-292.

8 . Oliveira JG, Alves PL and Vitória AP (2009) Alterations in chlorophyll a fluorescence, pigment concentrations and lipid peroxidation to chillingtemperature in coffee seedlings. Environmental and Experimental Botany. 67(1): 71-76.

9 . Ehlert B and Hincha DK (2008) Chlorophyll fluorescence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leaves. Plant Methods. 4(1): 1-7.

10 . Esra K, İŞLEK C and Üstün AS (2010) Effect of cold on protein, proline, phenolic compounds and chlorophyll content of two pepper (Capsicum annuum L.) varieties. Gazi University Journal of Science. 23(1): 1-6.

11 . Gross J (1991) Pigments in vegetables: chlorophylls and carotenoids. Van Nostrand Reinhold. New York.

12 . Hincha D, Sieg F, Bakaltcheva I, Koth H and Schmitt J (1996) Freeze-thaw damage to thylakoid membranes: specific protection by sugars and proteins. Advances in low-temperature biology (ed. PL Steponkus): 141-183.

13 . Hokanson SC and Finn CE (2000) Strawberry cultivar use in North America. HortTechnology. 10(1): 94-106.

14 . Ireland C, Baker N and Long S (1985) The role of carbon dioxide and oxygen in determining chlorophyll fluorescence quenching during leaf development. Planta. 165(4): 477-485.

15 . Khanizadeh S and Deell J (2001) Chlorophyll fluorescence: a new technique to screen for tolerance of strawberry flowers to spring frost. Small Fruits Review. 1(3): 61-67.

16 . Lang V, Mantyla E, Welin B, Sundberg B and Palva ET (1994) Alterations in water status, endogenous abscisic acid content, and expression of rab18 gene during the development of freezing tolerance in Arabidopsis thaliana. Plant Physiology. 104(4): 1341-1349.

17 . Lichtenthaler HK and Rinderle U (1988) The role of chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry. 19(sup1): S29-S85.

18 . Linden L, Palonen P and Hytönen T (2000) Evaluation of three methods to assess winter-hardiness of strawberry genotypes. Paper presented at the IV International Strawberry Symposium. P. 567.

19 . Lukoševičiūtė V, Rugienius R, Baniulis D, Savickienė N, Brazaitytė A, Ruzgas V and Šlepetienė A (2014). Characterization of cold acclimation and cold hardiness of strawberry in vitro and in vivo. (Doctora Thesis), Aleksandro Stulginskio universitetas. Akademija, Lietuva.

20 . Maxwell K and Johnson GN (2000) Chlorophyll fluorescence, a practical guide. Journal of Experimental Botany. 51(345): 659-668.

21 . McKenzie J, Weiser C and Burke M (1974) Effects of Red and Far Red Light on the Initiation of Cold Acclimation in Cornus stolonifera Michx. Plant Physiology. 53(6): 783-789.

22 . Miralles-Crespo J, Martínez-López JA, Franco-Leemhuis JA and Bañón-Arias S (2011) Determining freezing injury from changes in chlorophyll fluorescence in potted oleander plants. HortScience. 46(6): 895-900.

23 . Mishra A, Mishra KB, HöermillerII, Heyer AG and Nedbal L (2011) Chlorophyll fluorescence emission as a reporter on cold tolerance in Arabidopsis thaliana accessions. Plant Signaling and Behavior. 6(2): 301-310.

24 . Navari‐Izzo F, Quartacci MF, Pinzino C, Vecchia FD and Sgherri CL (1998) Thylakoid‐bound and stromal antioxidative enzymes in wheat treated with excess copper. Physiologia Plantarum. 104(4): 630-638.

25 . Nunes MES and Smith G (2003) Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Science. 43(4): 1349-1357.

26 . Ouellet F and Charron JB (2013) Cold acclimation and freezing tolerance in plants. eLS, John Wiley & Sons, Ltd. www.els.net: 1-6.

27 . Peguero-Pina JJ, Morales F and Gil-Pelegrín E (2008) Frost damage in Pinus sylvestris L. stems assessed by chlorophyll fluorescence in cortical bark chlorenchyma. Annals of Forest Science. 65(8): 1.

28 . Prášil I and Zámečnı́k J (1998) The use of a conductivity measurement method for assessing freezinginjury: I. Influence of leakage time, segment number, size and shape in a sample on evaluation of the degree of injury. Environmental and Experimental Botany. 40(1): 1-10.

29 . Pritts M, Worden K and Eames-Sheavly M (1989) Rowcover material and time ofapplication and removal affect ripening and yield of strawberries. Journal of the American Society for Horticultural Science. USA.

30 . Rahbarian P, Afsharmanesh G and Shirzadi M (2010) Effects of drought stress and manure on relative water content and cell membrane stability in dragonhead (Dracocephalum moldavica). Plant Echophysiology. 2(1): 13-19.

31 . Rajashekar CB and Panda M (2014) Water stress is a component of cold acclimation process essential for inducing full freezing tolerance in strawberry. Scientia Horticulturae. 174: 54-59.

32 . Ritchie SW, Nguyen HT and Holaday AS (1990) Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Science. 30(1): 105-111.

33 . Sairam R, Deshmukh P and Saxena D (1998) Role of antioxidant systems in wheat genotypes tolerance to water stress. Biologia Plantarum. 41(3): 387-394.

34 . Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Annual Review of Plant Physiology. 35(1): 543-584.

35 . Turner J, Tanino K and Stushnoff C (1993) Evaluation of low temperature hardiness of strawberry plants under field and controlled conditions. Canadian Journal of Plant Science. 73(4): 1123-1125.

36 . Wagner I (1992) Measurement of frost resistance in Rhododendron-hybrids. V International Rhododendron Conference. 364.

37 . Walker DA, Sivak MN, Prinsley RT and Cheesbrough JK (1983) Simultaneous measurement of oscillations in oxygen evolution and chlorophyll a fluorescence in leaf pieces. Plant Physiology. 73(3): 542-549.

38 . Yang C, Horn R and Paulsen H (2003) The light-harvesting chlorophyll a/b complex can be reconstituted in vitro from its completely unfolded apoprotein. Biochemistry.42(15): 4527-4533.