ریزازدیادی ختمی چینی در شرایط درون شیشه ای

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد باغبانی (گل و گیاهان زینتی) گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

2 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

چکیده

به منظور بررسی ریزازدیادی ختمی چینی در شرایط درون­شیشه­ای، سه آزمایش به صورت طرح ‌کاملاً تصادفی با 10 تکرار، در آزمایشگاه کشت بافت دانشکده کشاورزی دانشگاه شهید چمران اهواز در سال 1393 انجام گرفت. در آزمایش اول محیط پایه مناسب مشخص شد. آزمایش دوم بهترین غلظت هورمون BAP جهت القای شاخه و آزمایش سوم بهترین نوع و غلظت هورمون ریشه­زایی مشخص شد. مقایسه میانگین آزمایش اول نشان داد که بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره در محیط VS نسبت به سایر محیط­ها مشاهده شد. مقایسه میانگین آزمایش دوم نشان داد که غلظت 5/0 میلی­گرم بر لیتر هورمون BAP بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره نسبت به سایر غلظت­ها را داشت. مقایسه میانگین آزمایش سوم نشان داد که غلظت 2/0 میلی­گرم در لیتر هورمون  IBAبیشترین میانگین درصد ریشه­زایی، تعداد ریشه و طول ریشه نسبت به سایر غلظت­ها را داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Micropropagation of Hibiscus rosa-sinensis through tissue culture

نویسندگان [English]

  • Fatemeh Feizi 1
  • Mousa Mousavi 2
  • Mehrangiz Chehrazi 2
1 Graduate M.Sc. Student, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz. Ahvaz
2 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz
چکیده [English]

In order to investigate the in vitro micropropagation Hibiscus Rosa-sinensis three tests in a completely randomized design with 10 replications were performed in tissue culture laboratory Shahid Chamran University in 2014.The first experiment showed the perfect media.Second tests best consentration BAP for multiplication and third tests showed the best type and concentration rooting hormone. Compare average of first experiments showedthe highest average shoot length, number of leaves, fresh weight and dry weight was observed in VS than other media. Compare average of second experiments showedthe concentration of 5.0 mg per liter hormone BAP highest average shoot length, number of leaves, fresh weight and dry weight than other concentrations was observed.Compare third experiment showed that the average concentration of 2.0 mg per liter of IBA hormone highest percentage of rooting, number of roots and root length was compared to other concentrations.

کلیدواژه‌ها [English]

  • BAP
  • Hibiscus rosa-sinensis
  • Media VS
  • proliferation
  • Rotting
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