Encapsulation of two Iranian sunflower hybrid shoot tips and compared conversion and growth of synthetic seeds produced on different substrates

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

3 Associate Professor, Dry-land Agricultural Research Institute (DARI), Kermanshah, Iran

4 Former M.Sc. Student, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

Abstract

This investigation was carried out to determine the conversion and growth ability of artificial seeds derived from encapsulation of sunflower shoot tips in different substrates as factorial based completely randomized design with five replications, in tissue culture laboratory of Kermanshah Agriculture Research Center in 2013. Shoot tips excised from in vitro proliferated sunflower plantlets were encapsulated in three percent sodium alginate and 100 mM CaCl2.2H2O. Determination the effect of using nutrients and plant growth regulators, alginate matrix supplement with three different matrixes include distilled water, liquid MS medium and liquid MS medium whit tow mg/l BA and tow mg/l IAA. Synthetic seeds were transferred on hormone-free MS medium and two commercial substrates mixture of cocopeat, perlite (1:1) or cocopeat, perlite and peat moss (1:1:1) for generation and growth ability evaluation. Using of MS nutrients and plant growth regulators in calcium alginate beads significantly improved encapsulated explants growth. Maximum percentage response for conversion of synthetic seeds was 100 percent that was achieved from in vitro culture on MS medium and directly culture in ex vitro condition caused to decrease the conversion and growth them.

Keywords

References

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Applied Crop Breeding
Volume 4, Issue 1
August 2019
Pages 49-59

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