Synthetic amphiploid production from the crosses between some bread wheat cultivars and Aegilops triuncialis L

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Agronomy and Plant Breeding,Faculty of Agriculture, Universityof Kurdistan, Sanandaj, Iran

2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Universityof Kurdistan, Sanandaj, Iran

3 Assistant Professor, Department of Horticulture, Faculty of Agriculture, Universityof Kurdistan, Sanandaj, Iran

Abstract

Aegilops triuncialis L. (2n=4x=28; CtCtUtUt) is one of the tetraploid Aegilops species harboring valuable genes for resistance to many biotic and abiotic stresses. In the present study the bread wheat cultivars MV17, Navid, Omid, Pishgam, Zarin were crossed with an accession of Ae. triuncialis and the resulted F1 and F2 (obtained by the selfing of F1 plants) hybrids studied using the conventional cytogenetic methods. The crossability (seed set per pollinated floret) of whet cultivars was significantly different with an average of 46.24 percent. Chromosome counting confirmed the presence of 35 (n=5x=35; ABDUtCt) chromosomes in a sample of F1 seeds. The mean frequency of F2 seeds (per floret) was 3.54 percent. The F2 seeds fell in two distinct classes of shrank and smooth seeds. Chromosome counting in root tip cells revealed 40-70 chromosomes in a sample of F2 seeds, indicating the ocurance spontaneous amphiploid (2n=10x=70; AABBDDUtUtCtCt) formation in F2 generation. Induced polyploidy using colchicine was also successful in one of the treated F1 seeds, producing 70 chromosome seeds. Study of the metaphase I of the meiosis in F1 hybrids on average revealed 7 rod bivalents and 21 univalents in each Pollen Mother Cell (PMC). F1 seeds generally matured 30 days later than that of their wheat parents. The frequency of BC1F1 seeds resulting from crossing of F1 with the wheat parent was about 1.27 percent which was lower than F2 seed frequency.

Keywords

References

1.Alfares W, Bouguennec A, Balfourier F, Gay G, Bergès H, Vautrin S, Sourdille P, Bernard M and Feuillet C (2009) Fine mapping and marker development for the crossability gene SKr on chromosome 5BS of hexaploid wheat (Triticum aestivum L.). Genetics. 183: 469-481.
2. Arrigo N, Guadagnuolo R, Lappe S, Pasche S, Parisod C and Felber F (2011) Gene flow between wheat and wild relatives: empirical evidence from Aegilops geniculata, Ae. neglecta and Ae. triuncialis. Evolutionary Applications. 4: 685-695.
3. Badaeva E, Amosova A, Samatadze T, Zoshchuk S, Shostak N, Chikida N, Zelenin A, Raupp W, Friebe B and Gill B (2004) Genome differentiation in Aegilops. 4. Evolution of the U-genome cluster. Plant Systematics and Evolution. 246: 45-76.
4. Bretagnolle F and Thompson J (1995) Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytologist. 129: 1-22.
5. Chennaveeraiah M (1960) Karyomorphologic and cytotaxonomic studies in Aegilops. Acta Hort Gothoburg. 23: 85-178.
6.Claesson L, Kotimaki M and Bothmer Rv (1990) Crossability and chromosome pairing in some interspecific Triticum hybrids. Hereditas. 112: 49-55.
7.Evans LE (1964) Genome construction within the Triticeae I. The synthesis of hexaploids (2n = 42) having chromosomes of Agropyron and Aegilops in addition to the A and B genome of Triticum durum. Canadian Journal of Genetics and Cytology. 6: 19-28.
8.Farkas A, Molnár I, Dulai S, Rapi S, Oldal V, Cseh A, Kruppa K, Molnár-Láng M and Puertas M (2014) Increased micronutrient content (Zn, Mn) in the 3Mb (4B) wheat–Aegilops biuncialis substitution and 3Mb. 4BS translocation identified by GISH and FISH. Genome. 57: 61-67.
9.Fedak G and Jui PY (1982) Chromosomes of Chinese Spring wheat carrying genes for crossability with Betzes barley. Canadian Journal of Genetics and Cytology. 24: 227-233.
10.Gill B, Sharma H, Raupp W, Browder L, Hatchett J, Harvey T, Moseman J and Waines J (1985) Evaluation of Aegilops species for resistance to wheat powdery mildew, wheat leaf rust, Hessian fly, and greeenbug. Plant Disease. 69: 314-316.
11.Gill B and Friebe B (2002) Cytogenetics, phylogeny and evolution of cultivated wheats. Cytogenetics. 567.
12.Gong W, Li G, Zhou J, Li G, Liu C, Huang C, Zhao Z and Yang Z (2014) Cytogenetic and molecular markers for detecting Aegilops uniaristata chromosomes in a wheat background. Genome. 57: 1-9.
13.Kihara H and Lilienfeld F (1935) Weitere Untersuchungen an Aegilops X Triticum and Aegilops X Aegilops-Bastarden. Cytologia. 195-216.
14.Kilian B, Mammen K, Millet E, Sharma R, Graner A, Salamini F, Hammer K and Özkan H (2011) Aegilops. Wild Crop Relatives: Genomic and Breeding Resources. Springer.Pp 1-76.
15.Kimber G and Yen Y (1989) Hybrids involving wheat relatives and autotetraploid Triticum umbellulatum. Genome. 32: 1-5.
16.Kimber G and Tsunewaki K (1996) Genome symbols and plasma types in the wheat group. In Proceedings of the 7th International Wheat Genetics Symposium, 13-19 July 1988. Edited by Miller TE, Koebner RMD. Cambridge, England. Institute of Plant Science Research, Cambridge Laboratory, Trumpington, England: 1209-1210.
17.L David J, Benavente E, Bres-Patry C, Dusautoir J and Echaide M (2004) Are neopolyploids a likely route for a transgene walk to the wild? The Aegilops ovata× Triticum turgidum durum case. Biological Journal of the Linnean Society. 82: 503-510.
18.Liu D, Lan X, Yang Z, Zheng Y, Wei Y and Zhou Y (2002) A unique Aegilops tauschii genotype needless to immature embryo culture in cross with wheat. Acta Botanica Sinica. 44: 708-713.
19.Loureiro I, Escorial C, García-Baudin J and Chueca C (2009) Spontaneous wheat-Aegilops biuncialis, Ae. geniculata and Ae. triuncialis amphiploid production, a potential way of gene transference. Spanish Journal of Agricultural Research. 7: 614-620.
20.Martin-Sanchez J, Gomez-Colmenarejo M, Del Moral J, Sin E, Montes M, Gonzalez-Belinchon C, Lopez-Brana I and Delibes A (2003) A new Hessian fly resistance gene (H30) transferred from the wild grass Aegilops triuncialis to hexaploid wheat. Theoretical and Applied Genetics. 106: 1248-1255.
21.Mirzaghaderi G, Karimzadeh G, Hassani HS, Jalali-Javaran M and Baghizadeh A (2010) Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization. Biologia Plantarum. 54: 252-258.
22.Murai K and Tsunewaki K (1986) Molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. IV. CtDNA variation in Ae. triuncialis. Heredity. 57: 335-339.
23.Prazak R (2014) The role of Aegilops species in the origin and improvement of common wheat. Acta Agrobotanica. 66: 7-14.
24.Rajaram S, Varughese G, Abdalla O, Pfeiffer W and Van Ginkel M (1993) Accomplishments and challenges in wheat and triticale breeding at CIMMYT. In: Plant Breeding Abstracts.Pp. 131-139.
25.Ramsey J and Schemske DW (1998) Pathways, mechanisms, and rates of polyploid formation in flowering plants. Annual Review of Ecology and Systematics. 467-501.
26.Ramsey J and Schemske DW (2002) Neopolyploidy in flowering plants. Annual review of ecology and systematics. 589-639.
27.Rawat N, Tiwari VK, Neelam K, Randhawa GS, Chhuneja P, Singh K and Dhaliwal HS (2009 a) Development and characterization of Triticum aestivum–Aegilops kotschyi amphiploids with high grain iron and zinc contents. Plant Genetic Resources. 7: 271-280.
28.Romero M, Montes M, Sin E, Lopez-Brana I, Duce A, Martin-Sanchez J, Andres M and Delibes A (1998) A cereal cyst nematode (Heterodera avenae Woll.) resistance gene transferred from Aegilops triuncialis to hexaploid wheat. Theoretical and Applied Genetics. 96: 1135-1140.
29.Sharma H (1999) Embryo Rescue Following Wide Crosses. In: R. Hall (Editor),. Plant Cell Culture Protocols Methods In Molecular Biology Humana Press. Pp 293-307.
30.Sharma HC and Gill BS (1983) Current status of wide hybridization in wheat. Euphytica. 32: 17-31.
31.Tiwari VK, Rawat N, Neelam K, Kumar S, Randhawa GS and Dhaliwal HS (2010) Substitutions of 2S and 7U chromosomes of Aegilops kotschyi in wheat enhance grain iron and zinc concentration. Theoretical and Applied Genetics. 121: 259-269.
32.Valkoun J (2001) Wheat pre-breeding using wild progenitors. Wheat in a global environment. Springer.Pp. 699-707.
33.Vanichanon A, Blake N, Sherman J and Talbert L (2003) Multiple origins of allopolyploid Aegilops triuncialis. Theoretical and Applied Genetics. 106: 804-810.
34.Waines JG and Barnhart D (1992) Biosystematic research in Aegilops and Triticum. Hereditas. 116: 207-212.
35.Wang C-J, Zhang L-Q, Dai S-F, Zheng Y-L, Zhang H-G and Liu D-C (2010) Formation of unreduced gametes is impeded by homologous chromosome pairing in tetraploid Triticum turgidum× Aegilops tauschii hybrids. Euphytica. 175: 323-329.
36.Wang J, Luo MC, Chen Z, You FM, Wei Y, Zheng Y and Dvorak J (2013) Aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat D-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. New Phytologist. 198: 925-937.
37.Watanabe1 S, Endo1 TR and Nasuda1 S (2014) Chromosome 3B of Chinese Spring wheat is not a prerequisite for the gametocidal action of the Gc3-C1 gene. Wheat Information Service. 117: 1-3.
38.Yoshiya K, Watanabe N, Kuboyama T and Lapochkina I (2012) Genetic mapping of the gene for brittle rachis in a Triticum aestivum—Aegilops triuncialis introgression line. Genetic Resources and Crop Evolution. 59: 67-72.
39.You-wei Y, Zhang L-q, Yen Y, Zheng Y-l and Liu D-c (2010) Cytological Evidence on Meiotic Restitution in Pentaploid F1 Hybrids between Synthetic Hexaploid Wheat and Aegilops variabilis. Caryologia. 63: 354-358.
Applied Crop Breeding
Volume 2, Issue 2 - Serial Number 2
Autumn & Winter
October 2014
Pages 227-240

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