Evaluating reaction of rice (Oryza sativa L.) Mutant lines to salt stress at seedling stage

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Biotechnology and Plant Breeding, Faculty of Plant Sciences, Sari Agricultural Sciences and Natural Resources University (SANRU)

2 Associate Professor, Department of Biotechnology and Plant Breeding, Faculty of Plant Sciences, Sari Agricultural Sciences and Natural Resources University (SANRU)

3 Professor, Department of Biotechnology and Plant Breeding, Faculty of Plant Sciences, Sari Agricultural Sciences and Natural Resources University (SANRU)

4 Assistant Professor, Department of Biotechnology and Plant Breeding, Faculty of Plant Sciences, Agricultural Sciences and Natural Resources University (SANRU)

Abstract

In order to evaluate chemical mutation on cultivar mahali Tarrom’ in salt stress, seeds of rice cultivar ‘mahali Tarrom’ were treated with two chemical mutagens, ethyl methane sulfonate (14 mM) and sodium azide (two mM). In second generation, appropriate plants for economically important traits have been selected. seeding of selected plants were tested under salt stress in third generation using a factorial experiment with two factors based on completely randomized design with three replications at two salinity levels (zero and 12 dSm-1) were in hydroponic media. Results showed that two mutant lines obtained by treatment of ethyl methane sulfonate were stayed at tolerance group that acted better in comparison with the control (non-treated) under salt stress. However, two sensitive lines to salinity conditions were observed under mutagen treatment, while most of mutant lines in sodium azide treatment under salinity conditions were performed similar to control. Also, a number of sensitive lines to salinity were identified under treatment with this mutagen. 

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