Discrimination and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer SFF-SFR

Shahmoradi1, Masoud; Rasouli, Mousa; Hamidogli, Yusef; Imani, Ali; Footuhi Ghazvini, Reza

Discrimination and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer SFF-SFR

Document Type : Research Paper

Authors

Masoud Shahmoradi1 ¹

Mousa Rasouli ²

Yusef Hamidogli ³

Ali Imani ⁴

Reza Footuhi Ghazvini ³

¹ M.Sc. Student, Department of Landscape and Horticultural science, Faculty of Agriculture, Guilan University, Rasht, Iran

² Assistant Professor, Department of Horticulture, Faculty of Agriculture, Malayer University, Malayer, Iran

³ Department of Landscape and Horticultural Science, Faculty of Agriculture, Guilan University, Rasht, Iran

⁴ Associate Professor, Department of Horticultural, Seed and Plant Improvement Institute (SPII), Karaj 31587, Iran

Abstract

Self-incompatibility in almond and Prunus species is an important genetic trait that prevents self-fertilization. Self-incompatibility in almond is controlled gametophytically by the multiallelic S-locus. This study was done in order to identification and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer SFF-SFR.Some important morphological traits of parental crosses were evaluated using almond descriptor. Also, offspring’s (F₁) of five crosses including; A (Tuono × 101 Genotype), B (Supernova × 101 Genotype), C( Genco × Shahrood 21), D (Tuono × Shahrood 12) and E (Tuono × shahrood 17) were tested using PCR in order to DNA amplification and self-compatibility evaluation. Results of PCR method found that self-compatible progenies showed 449 bp band, while this band not observed in self-incompatible progenies. In addition, all self-incompatible progenies appeared no S₁ allele at any condition.

Keywords

almond

self-incompatibility

Sf-allele

S1-allele

SFF-SFR primer

PCR