Assessment of genetic diversity among some male and female pistachio (Pistacia vera L.) genotypes using RAPD marker

Document Type : Research Paper

Authors

1 M.Sc. Student of Horticulture, Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan - Iran

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan – Iran

3 Associate Professor, Department of Agronomy and Plant breeding, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan - Iran

4 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan – Iran

5 Instructor of Department Plant Biotechnology and Breeding, Pistachio Research Institute of Iran, Rafsanjan - Iran

Abstract

In order to study the genetic diversity among some male pistachio genotypes,16 male pistachio genotypes and 8 female pistachio cultivars were selected from Iranian Pistachio Research Institute (IPRI). 12 RAPD primers were used which totally produced 65 bands that among them 48 bands were polymorphic and 17 bands were monomorphic. Cluster analysis based on similarity matrix and Jaccard similarity coefficient and UPGMA method, at distance of 0.49 similarity, pistachio genotypes were situated into 5 main group. Study genetic diversity among male genotypes of pistachio using RAPD markers identified their genetic status so that divided Female genotypes as well as male genotypes and MO12 male genotype seems to be due to a number of less genetic similarity with female genotype can be used as an suitable pollinator for this cultivars which Research is needed in this context through controlled pollination occurs. According to the results of this research male pistachio genotypes had high diversity due to non grafting male genotypes in pistachio orchard, which make vast diversity in male genotypes in comparison with female cultivars.

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